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  • Title: Monoclonal antibody detection of IncF group plasmid-encoded TraT protein in clinical isolates of Escherichia coli.
    Author: Bitter-Suermann D, Peters H, Jürs M, Nehrbass R, Montenegro M, Timmis KN.
    Journal: Infect Immun; 1984 Nov; 46(2):308-13. PubMed ID: 6389341.
    Abstract:
    The TraT protein specified by IncF group plasmids mediates surface exclusion and bacterial resistance to the lethal activities of serum. In this study, an anti-TraT protein monoclonal antibody was generated which failed to react with TraT+ bacteria but which efficiently detected solubilized TraT protein in Western blots and in an enzyme-linked immunosorbent assay. Use of this antibody to screen clinical and nonclinical isolates of Escherichia coli for the production of TraT protein revealed its presence in a modest proportion (38%) of normal fecal strains, a significantly higher proportion of clinical strains (51 to 73%), and an even higher proportion (78 to 88%) of clinical strains concomitantly producing the K1 capsule, an important virulence factor of E. coli.
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