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  • Title: Trapping of the substrate-derived acyl enzyme intermediate of purified penicillin-binding protein 1a of Escherichia coli.
    Author: Amanuma H, Strominger JL.
    Journal: J Bacteriol; 1984 Nov; 160(2):824-5. PubMed ID: 6389502.
    Abstract:
    Purified penicillin-binding protein 1a of Escherichia coli formed an acyl enzyme intermediate with the highly reactive synthetic substrate diacetyl-L-lysyl-D-alanyl-D-lactate at acid pH, although in extremely low yields.
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