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  • Title: Bovine pneumonic pasteurellosis: effect of culture age of Pasteurella haemolytica used as a live vaccine.
    Author: Confer AW, Panciera RJ, Corstvet RE, Rummage JA, Fulton RW.
    Journal: Am J Vet Res; 1984 Dec; 45(12):2543-5. PubMed ID: 6395734.
    Abstract:
    Five experiments were conducted that compared aerosol immunization of calves with live Pasteurella haemolytica from logarithmic (6 hour) or stationary (20 to 22 hour) phase cultures. Calves were challenge exposed by transthoracic injection with P haemolytica. In 4 experiments, calves inoculated with 6-hour cultures had slightly lower mean lesion scores (indicating greater resistance to challenge exposure) than those inoculated with 20- to 22-hour cultures. High antibody titers, as detected by a quantitative fluorometric immunoassay or the indirect hemagglutination test, correlated directly with lung resistance (based on lesion scores) regardless of the age of the culture used as the immunogen.
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