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  • Title: The detection of Escherichia coli heat-labile enterotoxin by enzyme-linked immunosorbent assay.
    Author: Clarke DR, Harper IA, Thompson RG.
    Journal: J Infect; 1984 Nov; 9(3):244-51. PubMed ID: 6396337.
    Abstract:
    An enzyme linked immunosorbent assay (ELISA) technique was modified and used to detect heat-labile (LT) enterotoxigenic Escherichia coli (ETEC) from specimens of faeces sent to a routine diagnostic laboratory. Strains of LT ETEC were detected in eight of 250 (3.2%) diarrhoeal stools but were not found in 110 non-diarrhoeal stools. All eight patients found to have LT ETEC were travellers returning with diarrhoea. LT ETEC was the most common bacterial pathogen found (15.4%) in this group of patients, exceeding Campylobacter sp. (5.8%) and Salmonella sp. (7.7%). The ELISA technique was simple to perform and visual readings were found to be reliable.
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