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  • Title: An improved method for increasing the sensitivity of direct agglutination test (D.A.T.) to the detection of IgM and IgG anti-toxoplasma antibodies.
    Author: Filice G, Carnevale G, Meroni V, Gagliardi V, Castelli F, Carosi G.
    Journal: Boll Ist Sieroter Milan; 1984; 63(5):458-61. PubMed ID: 6397212.
    Abstract:
    The present investigation has shown that the antibody titers obtained by IgG-IFA correlate well with 2-ME-DA (correlation coefficient 0.927, p less than 0.001). These results are obtained when the antibody titers are expressed as IU/ml. Buffer without 2-ME and purification of IgM fractions by simple gel-filtration method seem to be necessary conditions for detecting IgM antibodies by DA test in adult patients. Therefore the DA with these micromodifications has a specificity and sensitivity similar to those of IgG and IgM-IFA tests and would be convenient for laboratories that perform large scale surveys during pregnancy.
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