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  • Title: An enhanced chemiluminescence enzyme immunoassay for serum progesterone.
    Author: Wang HX, Stott RA, Thorpe GH, Kricka LJ, Holder G, Rudd BT.
    Journal: Steroids; 1984 Oct; 44(4):317-28. PubMed ID: 6400058.
    Abstract:
    A competitive enhanced luminescent enzyme immunoassay for serum progesterone is described, which is based on a 11 alpha-hydroxyprogesterone 11-hemisuccinyl-horseradish peroxidase conjugate and a black polystyrene microtitre plate sensitised with anti-progesterone IgG. Bound label was determined using a mixture of 4-iodophenol, luminol and peroxide, and the light emitted from the wells of the plate quantitated using a luminescent plate reader. The assay was sensitive (detection limit 0.5 pg), precise (CV 2.7 - 9.0% in the concentration range 4.3-67.7 nM) and showed good correlation (r = 0.99) with a conventional radioimmunoassay.
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