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Title: Locus of the Pseudomonas aeruginosa toxin A gene. Author: Hanne LF, Howe TR, Iglewski BH. Journal: J Bacteriol; 1983 Apr; 154(1):383-6. PubMed ID: 6403508. Abstract: The gene for Pseudomonas aeruginosa toxin A has been mapped in the late region of the chromosome of strain PAO. Strain PAO-PR1, which produces parental levels of toxin A antigen that is enzymatically inactive and nontoxic, was used as the donor for R68.45 plasmid-mediated genetic exchange. Strain PAO-PR1 (toxA1) was mated with toxin A-producing strains, and exconjugates for selected prototrophic markers were tested for the transfer of toxA1. The toxA1 gene was located between cnu-9001 and pur-67 at approximately 85 min on the PAO chromosome.[Abstract] [Full Text] [Related] [New Search]