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  • Title: Reconstitution studies on the involvement of radiation-induced lipid peroxidation in damage to membrane enzymes.
    Author: Yukawa O, Nagatsuka S, Nakazawa T.
    Journal: Int J Radiat Biol Relat Stud Phys Chem Med; 1983 Apr; 43(4):391-8. PubMed ID: 6404847.
    Abstract:
    The effect of radiation on the drug-metabolizing enzyme system of microsomes, reconstituted with liposomes of microsomal phospholipids, NADPH-cytochrome P-450 reductase and cytochrome P-450, was examined to elucidate the role of lipid peroxidation of membranes in radiation-induced damage to membrane-bound enzymes. The reconstituted system of non-irradiated enzymes with irradiated liposomes showed a low activity of hexobarbital hydroxylation, whereas irradiated enzymes combined with non-irradiated liposomes exhibited an activity equal to that of unirradiated controls. Irradiation of liposomes caused a decrease in cytochrome P-450 content by destruction of the haem of cytochrome P-450 and also inhibited the binding capacity of cytochrome P-450 for hexobarbital. The relationship between radiation-induced lipid peroxidation and membrane-bound enzymes is discussed.
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