These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Involvement of both syn- and anti-dihydrodiol-epoxides in the binding of 7, 12-dimethylbenz(a)anthracene to DNA in mouse embryo cell cultures.
    Author: Sawicki JT, Moschel RC, Dipple A.
    Journal: Cancer Res; 1983 Jul; 43(7):3212-8. PubMed ID: 6406055.
    Abstract:
    7,12-Dimethylbenz(a)anthracene (DMBA):deoxyribonucleoside adducts, from enzymic hydrolyses of DNA from mouse embryo cells exposed in culture to [3H]DMBA, can be separated into two fractions on the basis of whether or not they bind to the phenyl boronic acid residues of Servacel DHB. This suggests that these two fractions of adducts are derived from anti and syn bay-region dihydrodiol-epoxides, respectively. The fluorescence spectra and interactions of the major components of these two fractions with borate ions substantiate this interpretation. These findings indicate that both syn- and anti-dihydrodiol-epoxides make a substantial contribution to DMBA binding to DNA in mouse embryo cells. For a given mouse embryo cell preparation, the relative contributions of each of these dihydrodiol-epoxides to DNA binding did not vary substantially with DMBA dose, with incubation time with DMBA, or in growing versus confluent cultures, although there were differences between one cell preparation and another.
    [Abstract] [Full Text] [Related] [New Search]