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  • Title: Human thymidine kinase. Purification and properties of the cytosolic enzyme of placenta.
    Author: Gan TE, Brumley JL, Van der Weyden MB.
    Journal: J Biol Chem; 1983 Jun 10; 258(11):7000-4. PubMed ID: 6406493.
    Abstract:
    Cytosolic thymidine kinase (EC 2.7.1.21) has been purified 5200-fold to apparent homogeneity from normal human placenta. The purification includes sequential affinity chromatography on blue-Sepharose and a thymidine column. The molecular weight of the enzyme determined by gel filtration and sucrose density ultracentrifugation is 92,000. The subunit molecular weight is 44,000, suggesting that the enzyme is a dimer in its native state. With isoelectric focusing, placental thymidine kinase demonstrated a single form with an isoelectric point of 9.1. The final purified enzyme preparation exhibits no immunological cross-reactivity with human mitochondrial thymidine kinase.
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