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  • Title: Immunonephelometry of apolipoprotein A-II in hyperlipoproteinemic serum.
    Author: Heuck CC, Erbe I, Frech K, Lohse P, Münscher G.
    Journal: Clin Chem; 1983 Jul; 29(7):1385-8. PubMed ID: 6407782.
    Abstract:
    Immunonephelometry of apolipoprotein A-II (apo A-II) in serum requires that factors responsible for nonspecific increases of light scattering be eliminated. Complete delipidation is not necessary for immunonephelometry of apo A-II; in fact, light scattering of immunocomplexes from delipidated HDL is greater than from isolated apo A-II. Therefore, using the isolated peptide as a standard does not suffice for measurements in serum. The concentration of protein influences the development of light scattering and must be considered in standardizing this measurement.
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