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  • Title: Kinetics of Ca2+ release shows interactions between the two classes of sites of troponin-C.
    Author: Wang CL, Leavis PC, Gergely J.
    Journal: J Biol Chem; 1983 Aug 10; 258(15):9175-7. PubMed ID: 6409902.
    Abstract:
    The kinetics of Ca2+-release from the two high affinity sites of troponin-C (TnC) was studied by the stopped flow technique following rapid mixing with either EDTA or excess TbCl3. The rate constants obtained by the two methods were 2.8 and 0.7 s-1, respectively. For the tryptic fragment of TnC that contains only the COOH-terminal half of the molecule, both methods generate rate constants of 2.2 s-1. These results are consistent with the interpretation that binding of Tb3+ to the Ca2+-specific sites reduces the rate of dissociation of Ca2+ from, and thereby enhances the affinity for, the Ca2+-Mg2+ sites; this, in turn, suggests interactions between the two halves of the TnC molecule.
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