These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Kinetic study on disappearance of gamma-butyrolactone-gamma-carbonyl-L-histidyl-L-prolinamide (DN-1417) from plasma using a radioimmunoassay for DN-1417 isobutylamide.
    Author: Oshima I, Saito S, Shiota K, Miyake A, Oka Y, Nakayama R.
    Journal: J Pharmacobiodyn; 1983 Mar; 6(3):202-8. PubMed ID: 6410041.
    Abstract:
    Kinetic study of disappearance of gamma-butyrolactone-gamma-carbonyl-L-histidyl-L-prolinamide (DN-1417) from human plasma has been performed by a sensitive and specific radioimmunoassay (RIA) using antiserum against DN-1417 isobutylamide. Antibody against N-[2-hydroxy-4-(isobutylcarbamoyl)butyryl]-L-histidyl-L-prolinamide (DN-isobutylamide) did not cross-react with any other TRH-related peptides tested. DN-isobutylamide was radioiodinated with 125I by the chloramine T method and the product had a specific activity of about 160 microCi/microgram. The sensitivity of the RIA was 50 pg per tube, and the intra- and inter-assay coefficients of variation at concentrations of 1-10 ng/ml were 7.0-11.6%. DN-1417 in the plasma was converted to a stable form, DN-isobutylamide, by incubation with isopropanol-isobutylamine at room temperature for 2 h. DN-Isobutylamide was separated from 2-hydroxy-4-carboxybutyryl-L-histidyl-L-prolinamide (DN-COOH) using a SEP-PAKTM C18 cartridge before RIa. The recovery of DN-isobutylamide from plasma was 86.2-98.2%. The half-life, volume of distribution and plasma clearance rate of DN-1417 examined in 6 normal male volunteers by intravenous injection of 500 micrograms DN-1417 citrate were 11.5 +/- 5.6 min in the first phase and 89.5 +/- 33.5 min in the second phase, 25.3 +/- 10.0 liters and 745.8 +/- 278.6 1/d, respectively. These results indicate that RIA of DN-isobutylamide is useful for analysing DN-1417 metabolism in humans.
    [Abstract] [Full Text] [Related] [New Search]