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Title: Chromatographic and fluorescence spectroscopic studies of individual 7,12-dimethylbenz[a]anthracene--deoxyribonucleoside adducts. Author: Moschel RC, Pigott MA, Costantino N, Dipple A. Journal: Carcinogenesis; 1983 Sep; 4(9):1201-4. PubMed ID: 6411379. Abstract: Compared with standard Sephadex LH-20 column chromatography, a newly developed high pressure liquid chromatographic separation of hydrocarbon deoxyribonucleoside adducts derived from the DNA of mouse embryo cell cultures exposed to 7,12-dimethylbenz[a]anthracene (DMBA) provides markedly superior resolution. Once resolved, the fluorescence spectroscopic properties of the three major DMBA--DNA adducts indicate that the fluorescence exhibited by adducts derived from a bay region syn dihydrodiol epoxide of DMBA differs subtly from that exhibited by adducts derived from the isomeric anti dihydrodiol epoxide.[Abstract] [Full Text] [Related] [New Search]