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  • Title: Triple fixation of Bacillus subtilis dormant spores.
    Author: Kozuka S, Tochikubo K.
    Journal: J Bacteriol; 1983 Oct; 156(1):409-13. PubMed ID: 6413496.
    Abstract:
    A triple-fixation method with a sequential application of 5% glutaraldehyde, 1% osmium tetroxide, and 2% potassium permanganate gave superior preservation of the ultrastructure of Bacillus subtilis dormant spores with a thick spore coat.
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