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  • Title: [Effect of glucose and ammonium nitrogen concentrations on riboxine biosynthesis].
    Author: Chagin BA, Kuzmenok VA, Biriukov VV.
    Journal: Prikl Biokhim Mikrobiol; 1983; 19(4):568-72. PubMed ID: 6413972.
    Abstract:
    The effect of glucose and ammonium nitrogen concentrations on the riboxine biosynthesis by the culture Bacillus subtilis GEN 265 was investigated. The purpose of the investigation was to optimize the process with respect to these parameters. The investigation was carried out in a 1 m3 fermenter. Glucose and ammonium nitrate were added for various fermentation times. It was shown that riboxine biosynthesis did not require glucose dosing in the course of the process, with the optimal concentration being 13-15%. Optimal concentrations of ammonium nitrogen were determined. It was found that the dose of 0.35 mg/ml provided a 24% increase in the yield.
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