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  • Title: The effect of defocusing on the electron microscopic image of the extended particle of pyocin R1.
    Author: Takeda Y, Kageyama M.
    Journal: J Biochem; 1983 Sep; 94(3):1013-6. PubMed ID: 6417116.
    Abstract:
    The effect of defocusing on the electron microscopic image of the extended particle of pyocin R1 was examined with the aid of optical diffraction. The results show that the through-focus image of the cross-striated particle changed to a fishbone-like image with a little under-focusing, which had been considered to be a transitional state during the sheath contraction of phage G. In the optical diffraction pattern of the defocused image, a strong meridional reflection corresponding to the distance of cross-striations in the through-focus image almost disappeared, supporting the change to the fishbone-like image on defocusing.
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