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  • Title: [Laboratory procedures with adenoviruses. X. Hemagglutination with glutaraldehyde-fixed erythrocytes].
    Author: Lang S, Wigand R.
    Journal: Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol; 1983 Sep; 255(2-3):202-8. PubMed ID: 6417943.
    Abstract:
    Erythrocyte preparations from man, rat, and monkey, fixed by glutaraldehyde (0.06%, final concentration, 30 min room temperature), remained agglutinable by various human adenoviruses. While incomplete adenovirus hemagglutinins from species of subgenera A, C, and E did not react with fixed blood cells, the numerous species of subgenera B and D, in which hemagglutination and hemagglutination-inhibition are essential for identification, did so. Fixed blood cells were also agglutinable by indirect hemagglutinins of adenovirus 9 and 11; hence, hemagglutination-enhancement tests with fixed blood cells are feasible. Fixed blood cells remained stable and agglutinable up to one year; they could also be lyophilized.
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