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  • Title: Simple and rapid high-pressure liquid chromatographic simultaneous determination of aspirin, salicylic acid, and salicyluric acid in plasma.
    Author: Peng GW, Gadalla MA, Smith V, Peng A, Chiou WL.
    Journal: J Pharm Sci; 1978 May; 67(5):710-2. PubMed ID: 641818.
    Abstract:
    A rapid and sensitive high-pressure liquid chromatographic assay was developed for aspirin, salicylic acid, and salicyluric acid in plasma. The procedure involves the solvent extraction of these compounds from plasma and separation using a reversed-phase column eluted by acidified aqueous acetonitrile. Small quantitites of aspirin can be assayed directly in the presence of a large quantity of salicylic acid. The assay is also free from blank interference.
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