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  • Title: Synthetic TN glycopeptide related to human glycophorin AM. High-field proton and carbon-13 nuclear magnetic resonance study.
    Author: Pavia AA, Ferrari B.
    Journal: Int J Pept Protein Res; 1983 Nov; 22(5):539-48. PubMed ID: 6418674.
    Abstract:
    Three 2-acetamido-2-deoxy-alpha-D-galactopyranoses attached to Ser2, Thr3 and Thr4 of the amino-terminal portion of glycophorin AM are responsible for the so-called TN blood group specificity. The corresponding glycopeptide H2N-Ser-Ser*-Thr*-Gly-OH obtained by a stepwise peptide coupling strategy was submitted to a detailed high-field nuclear magnetic resonance (n.m.r.) analysis. 13C-n.m.r. spectrum confirms the validity of previous assignments made on M sialo and asialoglycopeptides obtained by specific degradation of human glycophorin AM. In addition, the 400 MHz 1H-n.m.r. spectrum allowed most of the proton resonances to be assigned. A careful examination of the chemical shifts and coupling constants revealed some interesting features of the conformational properties of the GalNAc-Ser and GalNAc-Thr linkage as well as of the rotational isomerism of Thr and Ser side-chains. The data give conclusive evidence that high-field n.m.r. spectroscopy can be successfully used to gain structural and dynamic information on rather sophisticated glycopeptides.
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