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  • Title: Some factors affecting the quantitation of rheumatoid factors by enzyme immunoassay.
    Author: Togun RA, Brenchley P.
    Journal: J Immunol Methods; 1983 Dec 30; 65(3):343-50. PubMed ID: 6418821.
    Abstract:
    Some factors affecting the use of enzyme-labelled antibody to human IgM or enzyme-labelled human IgG for the detection and assay of IgM RFs by enzyme immunoassay have been investigated. IgM RF levels, obtained by standard radioimmunoassay techniques, showed a better agreement with those obtained with enzyme-labelled antibody than with enzyme-labelled human IgG. Immuno-adsorption studies using a sheep cell agglutination test (SCAT) confirmed a partial loss of antigenicity of the IgG molecules due to glutaraldehyde treatment, which increased with the length of time of exposure to glutaraldehyde.
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