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Title: Use of reducing compounds in the cultivation of Azospirillum sp. Author: Venkateswarlu B, Rao AV. Journal: Acta Microbiol Hung; 1983; 30(2):99-102. PubMed ID: 6419535. Abstract: Attempts were made to grow the micro-aerophilic N2-fixing bacterium Azospirillum sp. in complete liquid medium by incorporating some reducing agents. Ascorbic acid, glutathione and Na-thioglycollate stimulated while methylene blue inhibited the growth and N2-ase activity. In complete liquid medium, Na-thioglycollate and ascorbic acid increased the N2-ase activity with increasing concentration up to 800 micrograms/ml. With glutathione, growth of the bacterium was increased markedly but N2-ase activity was repressed below 200 micrograms/ml. The possibility of employing these compounds for the cultivation of Azospirillum in complete liquid medium seems to be indicated.[Abstract] [Full Text] [Related] [New Search]