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  • Title: Acrosin activity of canine spermatozoa as an index of cellular damage.
    Author: Froman DP, Amann RP, Riek PM, Olar TT.
    Journal: J Reprod Fertil; 1984 Jan; 70(1):301-8. PubMed ID: 6420555.
    Abstract:
    Eight experiments were performed to validate an extraction technique for canine acrosin and to quantitate the acrosin activity of fresh and frozen-thawed spermatozoa. Acrosin activity from fresh spermatozoa differed amongst dogs and was influenced by the interval since previous ejaculation. Freezing and thawing spermatozoa induced a loss of acrosin activity that differed with the extender in which the spermatozoa were frozen. The assay of acrosin activity, in conjunction with motility estimates, provides a more complete evaluation of the efficacy of seminal extenders in attenuating freezing injury than do motility estimates alone.
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