These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Antibody-free target cells stimulate chemiluminescence in polymorphonuclear leukocytes: an artifact due to mycoplasma contamination.
    Author: Peterhans E, Bertoni G, Köppel P, Wyler R, Keller R.
    Journal: Eur J Immunol; 1984 Feb; 14(2):201-3. PubMed ID: 6421603.
    Abstract:
    The generation of toxic oxygen species represents a significant mechanism in the killing of microorganisms and antibody-coated cells by phagocytic cells. We have investigated the possibility that antibody-free target cells stimulate reactive oxygen generation in polymorphonuclear leukocytes (PMNL) using the measurement of luminol-dependent chemiluminescence (CL). It was found that the induction of CL consistently correlated with a mycoplasma contamination of the target cells. Free mycoplasma organisms of two species found frequently as contaminants in cell culture also stimulated CL. Upon artificial infection with mycoplasma, cultured cells acquired the capacity to evoke CL generation in PMNL. Our experiments strongly suggest that the induction of reactive oxygen generation by antibody-free target cells is an artifact due to mycoplasma contamination of the target cell cultures.
    [Abstract] [Full Text] [Related] [New Search]