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  • Title: Determination of aflatoxin M1 in milk by liquid chromatography with fluorescence detection.
    Author: Cohen H, Lapointe M, Fremy JM.
    Journal: J Assoc Off Anal Chem; 1984; 67(1):49-51. PubMed ID: 6421796.
    Abstract:
    A liquid chromatographic (LC) method is proposed for the determination of aflatoxin M1 in milk. The method was successfully applied to both liquid whole and skim milk and also whole and skim milk powder. The samples are initially extracted with acetonitrile-water followed by purification using a silica gel cartridge and a C18 cartridge. Final analysis by LC was achieved using a radial compression module equipped with a 5 micron C18 column and a fluorescence detector. The method was successfully applied to samples at levels of 10 to 0.08 ppb added aflatoxin M1 with recoveries in the range of 70-98%.
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