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  • Title: [Expression of the genes for lysine biosynthesis of Bacillus subtilis in Escherichia coli cells].
    Author: Shevchenko TN, Okunev OV, Aleksieva ZM, Maliuta SS.
    Journal: Tsitol Genet; 1984; 18(1):58-60. PubMed ID: 6422596.
    Abstract:
    Hybrid plasmids pLRS33 and pLRB4 containing Bac. subtilis genes coding lysin biosynthesis were subjected to genetical analysis. It is shown that after pLRS33- and pLRB4- transformation of E. coli strains, auxotrophic relative to lysin and diaminopimelic acid, there occurs complementation of dapA, dapB, dapC, dapD, dapE, lysA mutations by plasmid pLRS33 and of dapC, dapB, lysA mutations by plasmid pLRB4. The plasmids are studied for their influence on the level of lysin and its precurror synthesis in E. coli strains.
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