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  • Title: Quantitative determination of valproic acid by means of gas chromatographic headspace analysis.
    Author: Degel F, Heidrich R, Schmid RD, Weidemann G.
    Journal: Clin Chim Acta; 1984 May 16; 139(1):29-36. PubMed ID: 6426826.
    Abstract:
    We have developed a simple but accurate procedure to monitor, by means of gas chromatographic headspace analysis, the concentration of valproic acid (VPA) in serum. Unlike previously described gas chromatographic methods, a preanalytical separation step is unnecessary. Irreversible sample loss, a pitfall in gas chromatographic extraction procedures caused by evaporation during the extraction process of this relatively volatile substance, is avoided. After acidifying and adding internal standard (2- ethylbutanoic acid), the serum sample is sealed hermetically in a special headspace tube. After heating, a vapour phase fraction is injected into the chromatographic column. This method reduces sample preparation time to a few minutes. Analysis time is only three minutes, and the method can easily be automated. Contamination of chromatographic columns by higher molecular mass serum ingredients is avoided.
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