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  • Title: Sexual agglutinin of mating-type minus gametes in Chlamydomonas reinhardii. I. Loss and recovery of agglutinability of gametes treated with EDTA.
    Author: Saito T, Matsuda Y.
    Journal: Exp Cell Res; 1984 Jun; 152(2):322-30. PubMed ID: 6426985.
    Abstract:
    The effect of EDTA on the mating-type-specific agglutinins located on the flagellar surfaces of Chlamydomonas reinhardii gametes was investigated. The mating-type minus (mt-) gametes lost their agglutinability without apparent loss of motility soon after addition of EDTA at low concentrations (1-2 mM). At the same time, the cells released into the medium agglutinins which can elicit agglutinative responses of mating-type plus (mt+) gametes specifically. When EDTA was neutralized with Mg2+ or removed by centrifugation, the mt- cells quickly replaced agglutinins by protein synthesis: the recovery process was sensitive to cycloheximide, but not to tunicamycin. The EDTA-treated mt+ gametes lost their agglutinins much more slowly than the mt- gametes. The replacement of mt+ agglutinins was inhibited by both cycloheximide and tunicamycin.
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