These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Interspecies somatic hybrid cultures from human epidermal cells and 3T3-4E mouse fibroblasts].
    Author: Chardonnet Y, Lizard G, Léry X, Schmitt D, Patet J, Viac J.
    Journal: C R Acad Sci III; 1984; 298(16):445-8. PubMed ID: 6428717.
    Abstract:
    Interspecific somatic hybrids were obtained by fusion of adult human epidermal cells with Mouse fibroblasts 3T3-4E, deficient in thymidine kinase. These hybrids were identified by their morphology and by the presence of markers from the parental cells. Some characters of keratinocytes such as keratin subunits 50 and 51 K were present in primary cultures and disappeared after serial passages, whereas bullous pemphigoid basement membrane zone antigens persisted for at least 20 passages. At the 7th passage, a metacentric chromosome, and more often a submetacentric chromosome, presumably of human origin, were observed in some cells.
    [Abstract] [Full Text] [Related] [New Search]