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  • Title: Assay of galactosyltransferase by high-performance liquid chromatography.
    Author: Hymes AJ, Mullinax F.
    Journal: Anal Biochem; 1984 May 15; 139(1):68-72. PubMed ID: 6430123.
    Abstract:
    Galactosyltransferase catalyzes transfer of galactose from UDP-galactose to glucose or N-acetylglucosamine with resultant formation of galactosides and UDP. In this new assay galactosyltransferase activity is measured by determining UDP by isocratic high-performance liquid chromatography on an amino-bonded column monitored spectrophotometrically. Concurrently, unreacted UDP-galactose and breakdown products arising from UDP-galactose (UMP and uridine) are also determined. The new technique does not require radioactive substrates, permits usage of saturating concentrations of UDP-galactose, and provides monitoring of side reactions.
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