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  • Title: Pad-plate diffusion assay for virginiamycin in mash-type feeds.
    Author: Katz SE, Katz JM, Miller JA, Wang R, Shapiro R.
    Journal: J Assoc Off Anal Chem; 1984; 67(3):569-72. PubMed ID: 6430863.
    Abstract:
    An assay procedure has been developed for virginiamycin in mash-type feeds which includes removing fat interferences from the feeds by a rapid petroleum ether wash, a citric acid extraction, an acetone extraction, and the use of the pad-plate diffusion system. The procedure eliminates the need for compensatory curves prepared from feeds of the same or similar composition. Recoveries from 10 laboratory-prepared feeds containing 11.0 micrograms virginiamycin/g averaged 89.3% with a coefficient of variation (CV) of 5.76%. Recoveries from a laboratory-prepared feed containing 13.2 micrograms virginiamycin/g averaged 89.9% (CV 9.92%). The procedure is simple, reasonably accurate, and precise.
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