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  • Title: The stoichiometry of A23187- and X537A-mediated calcium ion transport across lipid bilayers.
    Author: Blau L, Stern RB, Bittman R.
    Journal: Biochim Biophys Acta; 1984 Nov 21; 778(1):219-23. PubMed ID: 6437447.
    Abstract:
    Initial rates of ionophore-mediated Ca2+ transport across egg phosphatidylcholine bilayers of large unilamellar vesicles were measured using the absorbance change of arsenazo III at 650 nm as an indicator of Ca2+ translocation. A23187 induced the movement of Ca2+ in a 2:1 ionophore: Ca2+ complex, whereas its methyl ester (CH3A23187) and X537A mediated Ca2+ movement in a 1:1 ionophore: Ca2+ complex. The relative potencies of these ionophores in transporting Ca2+ across lipid membranes were A23187 much greater than X537A greater than CH3A23187.
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