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  • Title: A simple method for quantitative measurement of C3d in human plasma.
    Author: Bhakdi S, Roth M, Nürnberger W.
    Journal: J Immunol Methods; 1984 Nov 16; 74(1):79-86. PubMed ID: 6438236.
    Abstract:
    We describe a simple and reliable method for quantitating C3d in human plasma. The method rests on the finding that native C3 and its activation/inactivation product C3c bind to Concanavalin A, whereas C3d does not. Rocket affinoimmunoelectrophoresis with Con-A Sepharose incorporated into an intermediate gel permits quantitation of free C3d in 2-20 microliters aliquots of EDTA-plasma without any manipulation prior to sample application. Using this method, we found that the level of circulating C3d in plasma of 30 healthy donors was usually well below 3% of maximally convertable C3d.
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