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  • Title: Glycosidic bond rearrangements in isomeric xylobioses by yeast xylan-degrading enzymes.
    Author: Biely P, Petráková E.
    Journal: FEBS Lett; 1984 Dec 10; 178(2):323-6. PubMed ID: 6439578.
    Abstract:
    The cells of Cryptococcus albidus induced for xylan-degrading enzymes are capable of transforming 1,2-beta-xylobiose and 1,3-beta-xylobiose into 1,4-beta-xylobiose, the natural inducer. The conversion involves transglycosylation and hydrolysis catalyzed by beta-xylosidase and beta-xylanase. A probable intermediate of the conversion of 1,2-beta-xylobiose was isolated and identified as a trisaccharide, 4-O-beta-xylopyranosyl-2-O-beta-xylopyranosyl-D-xylopyran ose. The trisaccharide is cleaved by purified endo-1,4-beta-xylanase of C. albidus mainly at the 1,2-beta-linkage yielding xylose and 1,4-beta-xylobiose.
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