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  • Title: Effects of A23187 and Ca2+ on volume- and thiol-stimulated, ouabain-resistant K+C1- fluxes in low K+ fluxes in low K+ sheep erythrocytes.
    Author: Lauf PK, Mangor-Jensen A.
    Journal: Biochem Biophys Res Commun; 1984 Dec 14; 125(2):790-6. PubMed ID: 6440562.
    Abstract:
    Ouabain-resistant (OR), C1- -dependent K+ (K+C1-) transport measured by Rb+ influx in isosmotic and anisosmotic media was stimulated by the Ca2+ ionophore A23187 and EGTA (ethylene-glycol-tetracetic acid) in low K+ (LK) but not in high K+ (HK) sheep red cells. Increasing external Ca2+ concentrations, [Ca2+]o, from about 10(-7) to 10(-3)M in presence of A23187 and in absence of EGTA inhibited OR Rb+ influx, in LK red cells osmotically shrunken or swollen as well as treated with the thiol reagent N-ethylmaleimide (NEM). Hence the volume- and the NEM-stimulated K+C1- transport system in LK cells can be experimentally modulated by cellular Ca2+ or other Me2+, which may interact with sites on the K+C1- transporter under the control of membrane sulfhydryl (SH) groups.
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