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  • Title: Identification of a disialoganglioside (GD1a) containing terminal N-acetyl-9-O-acetylneuraminic acid in rat erythrocytes.
    Author: Gowda DC, Reuter G, Shukla AK, Schauer R.
    Journal: Hoppe Seylers Z Physiol Chem; 1984 Oct; 365(10):1247-53. PubMed ID: 6440851.
    Abstract:
    Gangliosides containing 350 micrograms of sialic acids were isolated from 2.85 X 10(11) rat erythrocytes and found to be mainly composed of GD1a and an unknown alkali-labile species which was converted to GD1a after treatment with ammonia. Smaller amounts of GM1 and Fuc-GM1 were also present. Identification of the sialic acids of the novel species by thin-layer chromatography, high performance liquid chromatography and gas-liquid chromatography--mass spectrometry revealed the presence of both N-acetylneuraminic acid and N-acetyl-9-O-acetyl-neuraminic acid in about equimolar amounts. Incubation of the isolated ganglioside with Vibrio cholerae sialidase released N-acetyl-9-O-acetyl-neuraminic acid. Non O-acetylated GM1 was identified as the only remaining ganglioside by thin-layer chromatography. Thus this novel ganglioside has the following structure: Neu5,9Ac2 alpha 2-3Gal beta 1-3GalNAc beta 1-4(Neu5Ac alpha-2-3)Gal beta 1-4Glc beta 1-1'Cer.
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