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  • Title: Studies of the structure-function relationships of Neurospora pyruvate kinase: denaturation by urea and the effect of ligands on the refolding and renaturation process.
    Author: O'Brien MD, Kapoor M.
    Journal: Int J Biochem; 1980; 11(2):107-16. PubMed ID: 6444599.
    Abstract:
    1. The renaturation of Neurospora pyruvate kinase following denaturation with urea was investigated. 2. The substrates, phosphoenol pyruvate and adenosine diphosphate, were observed to stabilize the enzyme against urea-induced structural disorganization. 3. It was demonstrated that for refolding/reactivation of the denatured protein the allosteric activator, fructose-1,6-diphosphate and a sulphydryl protectant are required. 4. The enzyme recovered following renaturation showed complete immunological identity with the native enzyme in Ouchterlony double diffusion tests.
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