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  • Title: The simultaneous identification of seminal acid phosphatase and phosphoglucomutase by starch gel electrophoresis.
    Author: Linde HG, Molnar KE.
    Journal: J Forensic Sci; 1980 Jan; 25(1):113-7. PubMed ID: 6446593.
    Abstract:
    Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.
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