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  • Title: [Allosteric properties of CF1-ATPase form chloroplasts].
    Author: Mal'ian AN.
    Journal: Biokhimiia; 1980 Oct; 45(10):1731-9. PubMed ID: 6453621.
    Abstract:
    The inhibiting effect of ADP and Mg2+ on CF1-ATPase from chloroplasts depending on their concentration, pH and the presence of stimulating agents of various origin was studied. It was shown that the low Mg-dependent activity of the soluble enzyme is due to non-competitive inhibition of the reaction by Mg2+ in the presence of ADP. The CF1-ATPase stimulators lower the inhibiting effect, thus allowing to detect the "true" Mg-dependent activity of the enzyme. The data obtained are indicative of the existence of Mg2+- and ADP-specific sitein the enzyme, which controls its catalytic activity. The properties and possible role of this site in photophosphorylation are discussed.
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