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  • Title: Studies on the male antifertility agent-gossypol acetic acid. II. Effect of gossypol acetic acid on the motility and ATPase activity of human spermatozoa.
    Author: Kalla NR, Vasudev M.
    Journal: Andrologia; 1981; 13(2):95-8. PubMed ID: 6454365.
    Abstract:
    A marked reduction in the motility of the human spermatozoa was observed when spermatozoa were incubated with 10 and 100 microgram of gossypol acetic acid for different time period at 37 degrees C; the motility was reduced from 83% to 4% after 210 minutes (P less than 0.001). A significant decrease in the activities of Ca++ and Mg++ activated ATPase in the spermatozoa was observed after gossypol treatment (P less than 0.005). Changes in the zinc++ concentration in the spermatozoa after gossypol treatment were, however, not significant. The present investigations suggest that inhibition of the sperm motility after gossypol treatment may be due to the inhibition of the ATPase activity in the spermatozoa. Freshly ejaculated human semen samples of proven motility were studied to test the hypothesis that inhibition of sperm motility after gossypol treatment (a polyphenolic pigment isolated from cottonseed) is caused by decreased adenosine triphosphatase (ATPase) activity and/or imbalance in the molecules of biological significance. When sperm were incubated with 10 and 100 mcg of gossypol acetic acid for different time periods at 37 degrees centigrade, a marked reduction in motility was abserved. The motility was reduced from 83-4% after 210 minutes (P .001). Also, a significant decrease in the activities of calcium ion and magnesium ion activated ATPase in the sperm was observed after incubation in gossypol (P .005). Zinc ion concentration also showed a change, but it was not statistically significant. These data indicate that sperm motility inhibition after gossypol treatment is due to inhibition of ATPase activity in the sperm. This research was conducted in India.
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