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  • Title: [Characteristics of the primary and secondary DNA structure of staphylococcal phage SB-1].
    Author: Andriashvili IA, Kvachadze LI, Adamiia RSh, Tushishvili DG, Kalandarishvili LL.
    Journal: Vopr Virusol; 1981; (1):100-3. PubMed ID: 6455010.
    Abstract:
    Equilibrium centrifugation, spectral analysis of thermal denaturation and direct chemical determinations showed staphylococcal phage Sb-I DNA to be characterized by a standard set of nitric bases (28.5 mol.2./% G-C). No abnormal bases or other extracomponents were found. From the differential spectral analysis of melting interval it is concluded that G-C pairs are distributed along DNA molecule in a Gauss type. Spectrophotometric and thermodynamic parameters of melting show phage Sv-I DNA to have a typical double-stranded structure. DNA is characterized by enthalpies of conformational transitions of spiral=glome delta H=11.4 cal/g and delta H = 9.7 cal/g for 1 x SSC and 0.1 x SSC diluents, respectively.
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