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  • Title: [The significance of mixed lymphocyte culture and mitogen-stimulated culture for kidney transplantation (author's transl)].
    Author: Hajek-Rosenmayr A, Wolf A.
    Journal: Wien Klin Wochenschr; 1981 Jul 10; 93(14):457-60. PubMed ID: 6455859.
    Abstract:
    In order to detect HLA-D identity or incompatibility, MLC tests were performed in 21 recipient-donor combinations before kidney transplantation (20 cadaver donors, one living related donor). In 20 cases, the MLC reaction was positive (R.R. greater than 10%). 13 of these patients rejected the transplant during the first three months after transplantation. One case was observed in which the MLC reaction between recipient and donor was negative. In this case, the graft has been functioning for more than a year. The lymphocytes of 7 kidney graft recipients were investigated in mitogen-stimulated lymphocyte cultures. In some cases, rejection crises were recognized in the test on the basis of a significantly enhanced reactivity of mitogen-stimulated lymphocytes. When no increase of proliferation was detected in the cultures, a rejection crisis became most improbable. One crisis failed to be detected and 6 false positive reactions occurred.
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