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  • Title: Efficiency of phage protein synthesis in lambda-infected E. coli minicells.
    Author: Lipińska B, Podhajska A, Taylor K.
    Journal: Acta Biochim Pol; 1980; 27(3-4):273-80. PubMed ID: 6455894.
    Abstract:
    Phage lambda major head protein, the gene E product, has been identified among other phage proteins synthesized in lambda-infected Escherichia coli minicells, separated by SDS-acrylamide gel electrophoresis. On stained gels, the same protein has also been detected among total (bacterial and phage) proteins of lambda-infected minicells. The contribution of lambda proteins to the total protein content of lambda-infected minicells was found to be about 12% following 30 min lambda-infection. The inhibition of lambda early protein synthesis (shown by other authors in nucleate bacterial cells) practically does not occur in minicells; this may be the reason of the observed high efficiency of phage protein synthesis.
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