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  • Title: TSH stimulates 32P-labeling of thyroid nuclear HMG 14, a protein associated with actively transcribed chromatin.
    Author: Cooper E, Palmer RJ, Spaulding SW.
    Journal: Endocrinology; 1982 Apr; 110(4):1459-61. PubMed ID: 6460611.
    Abstract:
    Thyroid slices were incubated with 32Pi with or without TSH. 32P-labeling of acid-soluble nuclear proteins was then examined by two-dimensional polyacrylamide gel electrophoresis and autoradiography. We found that TSH enhanced the labeling of the high mobility group protein HMG 14, a protein that is preferentially associated with actively transcribed chromatin. This observation suggests that changes in HMG 14 phosphorylation may be involved in mediating TSH-induced effects on the structure and function of active chromatin.
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