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  • Title: [Heterogeneity of leghemoglobin from yellow lupine nodules].
    Author: Kudriavtseva NN, Borodenko LI, Krasnobaeva NN, Zhizhevskaia GIa.
    Journal: Biokhimiia; 1978 Feb; 43(2):321-6. PubMed ID: 647081.
    Abstract:
    A possibility is demonstrated to separate summary lupine leghemoglobins (which are salted out within 55--90% of ammonium sulphate saturation) into Lb I and Lb II components by means of ionic exchange chromatography on DEAE-cellulose. Lb I is eluted at lower ionic strength buffer than LbII, and differs from the latter in the form and the size of crystals. Both components have the same electrophoretic mobility and contain N-terminal glycine. LbII and LbI precipitate under gradual salting out within 55--75% and 78--90% of saturation respectively.
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