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  • Title: Demonstration of prolactin at the ultrastructural level in a pituitary adenoma by the use of colloidal gold labelling.
    Author: Brion JP, Couck AM, Flament-Durand J, D'Haens J, Mockel J.
    Journal: J Submicrosc Cytol; 1984 Jul; 16(3):555-60. PubMed ID: 6471148.
    Abstract:
    A prolactin-secreting pituitary adenoma was removed in a 38-year-old man. The tumor was classified as a chromophobe adenoma on the basis of tinctorial staining, but immunocytochemistry revealed that cells reacted with anti-PRL serum. Electron microscopy showed secretory granules and evidence of high metabolic activity (well developed Golgi apparatus and rough endoplasmic reticulum). Immunoelectron microscopy by the immunogold staining method allowed a clear definition of prolactin containing granules.
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