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  • Title: Estimation of hydrogen peroxide in plant extracts using titanium(IV).
    Author: Patterson BD, MacRae EA, Ferguson IB.
    Journal: Anal Biochem; 1984 Jun; 139(2):487-92. PubMed ID: 6476384.
    Abstract:
    Methods for the estimation of hydrogen peroxide in acetone extracts using titanium(IV) are likely to overestimate hydrogen peroxide when applied to plant leaves. Pigments appear to co-precipitate with the titanium complex and cannot be removed by washing with solvents. Fluoride, which specifically removes the color of the titanium-peroxide complex, removes only some of the color from the reactions with plant extracts. This problem has been avoided by extracting tissues with trichloroacetic acid, and measuring peroxide against catalase-treated blanks by its reaction with the complex of titanium(IV) with 4-(2-pyridylazo) resorcinol. Levels of hydrogen peroxide in leaves of a variety of species were found to range from about 0.1 to 0.6 mumol X g-1.
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