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  • Title: On the size-dependent disintegration of small unilamellar phosphatidylcholine vesicles in rat plasma. Evidence of complete loss of vesicle structure.
    Author: Scherphof G, Morselt H.
    Journal: Biochem J; 1984 Jul 15; 221(2):423-9. PubMed ID: 6477475.
    Abstract:
    The destruction of small unilamellar egg phosphatidylcholine vesicles in rat plasma was monitored by measuring release of encapsulated 125I-poly(vinylpyrrolidone) or carboxyfluorescein and by determining transfer of radiolabelled phosphatidylcholine to plasma lipoproteins by means of gel filtration. The susceptibility of the vesicles to the destructive action of plasma increased with decreasing vesicle size, as observed by incubating plasma with individual fractions constituting the small-vesicle peak on Sepharose CL-2B. This results in selective destruction of small vesicles when heterogeneous vesicle populations are incubated with plasma. Samples of homogeneous vesicle populations were incubated with a wide range of plasma concentrations, which resulted in extents of solute and phospholipid release ranging from 10 to 90%. When the extents of solute release were plotted against the extents of lipid release a linear, virtually 1:1, relationship was found, for both carboxyfluorescein and poly(vinylpyrrolidone) as the solute. This suggests that the release of solutes from small unilamellar phosphatidylcholine vesicles as a result of their interaction with plasma (lipo)proteins involves the total destruction of a fraction of the vesicles, the magnitude of which is determined by the vesicle: plasma ratio. Our results argue against a previously presented view suggesting that the interaction between such vesicles and plasma results in the formation of pores through which encapsulated solutes diffuse at Mr-dependent rates [Kirby & Gregoriadis (1981) Biochem. J. 199, 251-254]. The discrepancies between the two studies in observations as well as in interpretation are discussed.
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