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  • Title: Separation of bovine brain mono- and diacylglycerol lipases by heparin sepharose affinity chromatography.
    Author: Farooqui AA, Taylor WA, Horrocks LA.
    Journal: Biochem Biophys Res Commun; 1984 Aug 16; 122(3):1241-6. PubMed ID: 6477559.
    Abstract:
    Mono- and diacylglycerol lipases are differentially inhibited by heparin. No other glycosaminoglycan resembles heparin in this respect. Mono- and diacylglycerol lipases can be separated by heparin Sepharose affinity chromatography. Diacylglycerol lipase was completely retained on a heparin--Sepharose column and was eluted with either 0.5 M NaCl or 2-5 mg/ml heparin, whereas monoacylglycerol lipase was recovered in the washings. Adenosine phosphates markedly affected the activity of diacylglycerol lipase in a concentration dependent manner. ATP was the most potent inhibitor followed by ADP. AMP had no effect and cAMP slightly stimulated the diacylglycerol lipase.
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