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  • Title: The beta-major and beta-minor globin genes in murine erythroleukemia cells replicate during the same early interval of the S phase.
    Author: Braunstein JD, Schildkraut CL.
    Journal: Biochem Biophys Res Commun; 1984 Aug 30; 123(1):108-13. PubMed ID: 6477576.
    Abstract:
    The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the beta-major globin gene and the 14 kb EcoRI segment containing the beta-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a beta-globin cDNA probe. Quantitation showed that both beta-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.
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